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Current Assays: 2009

ACTH

Adiponectin

Adrenalin

Androstenedione

Basic Fibroblast Growth Factor (bFGF)

Bone Specific Alkaline Phosphatase

Chondrex

C-peptide

CMV igG

Cortisol

Creatinine

C-reactive Protein

CrossLaps

Dehydro Epiandrosterone

Dehydro Epiandrosterone-sulfate (DHEA-S)

Deoxypyridinoline crosslinks (DPD)

Epinephrine

Erythropioetin

Estradiol

Estrone

FGF-23 (intact)

Follicle Stimulating Hormone (FSH)

Free Fatty Acids (non-esterified)

Gastric Inhibitory Protein (GIP)

Glucagon-Like Peptide (GLP)

Glucose

Ghrelin

Growth Hormone

GH ultra sensitive

25-Hydroxyvitamin D

ICAM, soluble

ICTP

Insulin

Insulin ultra sensitive

Insulin-like Growth Factor-I (IGF-1)

Interferon-gamma

Interleukens (IL-6, -8, -10, -12, -13)

IL-1 receptor agonist

IL-6 receptor

Leptin

Leptin receptor

Lipid Panel

Cholesterol (total)
HDL
LDL
VLDL
Triglycerides

LP(a)

Luteinizing Hormone (LH)

MCP-1

MIF

MMP-9

Myeloperoxidase

Norepinephrine

NTX

NTXS

Osteocalcin

Osteoprotegerin

PAI-1/tPA

Parathyroid Hormone (intact, iPTH)

PIIINP

PINP

Pro-BNP

PICP (PICP)

Pro-insulin

Prolactin

Resistin

Serum Amyloid A

Sex hormone binding globulin (SHBG)

Selectin, soluble P-selectin

Selectin, soluble E-selectin

SGP130

Testosterone (free or total)

Thyroid Stimulating Hormone (TSH)

Triiodothyronine (T3)

Thyroxin (T4)

Tumor Necrosis Factor alpha (TNFalpha)

TNFRI

TNFRII

tPA

vWF

Androstenedione

RIA
Supplier: ALPCO, Windham, NH

Linear range: 1 - 120 ng/ml
Sensitivity: 0.9 ng/ml
Specimen requirements: 100 µl of serum/plasma

Intra-assay variation (CV%) = n.d.
Inter-assay variation (CV%) = n.d.

Expected values *

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Bone Specific Alkaline Phosphatase

IRMA
Supplier: Hybritech Inc., San Diego, CA

Linear range: 15 - 120 ng/ml
Sensitivity: 2.0 ng/ml
Specimen requirements: 100 µl of serum in duplicate

Intra-assay variation (CV%) = 5.49 (n=22)
Inter-assay variation (CV%) = 5.83 (n=31)

Expected values *

age (years) n (male/female) BAP (ng/ml)

20-88 353 (180/173) 11.1+4.4

* J. Clin. Endocrinol. Metab. 77:1046-1053, 1993.

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Chondrex

ELISA
Supplier: Metra Biosystems, Inc. Palo Alto,CA

Linear range: 20 - 300 ng/ml
Sensitivity: 20 ng/ml
Specimen requirements: 20 µl of serum in duplicate

Intra-assay variation (CV%) = 4.22 (n=6)
Inter-assay variation (CV%) = 0 (n=0)

Expected values *

ng/ml

males
24-125

females
25-93

* Values given by kit supplier

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Cortisol

Serum Cortisol
RIA
Supplier: DSL, Inc. Webstar, TX.

Linear range: 0.5 - 60 µg/dl
Sensitivity: 0.11 µg/dl
Specimen requirements: 25µl of serum/plasma in duplicate

Intra-assay variation (CV%) = 6.71 (n=5)
Inter-assay variation (CV%) =

Expected values *

µg/dl

a.m.
5 - 25

p.m.
2 - 12

* Values given by kit supplier

Urinary Cortisol (by extraction)
RIA
Supplier: DSL, Inc. Webstar, TX.

Linear range: 0.5 - 60 µg/dl
Sensitivity: 0.11 µg/dl
Specimen requirements: 250µl of urine in duplicate
Antibody:

Intra-assay variation (CV%) = 4.61 (n=5)
Inter-assay variation (CV%) = 8.01 (n=3)

Expected values * µg/dl 0 - 10 years 2 - 27 µg/24h 11 - 17 years 1 - 55 µg/24h >18 years 5 - 55 µg/24h

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Creatinine

Serum Creatinine

Supplier: Beckman Diagnostics, Fullerton, CA 92634-3100

Method of Measurement:Jaffe Rate (alkaline picrate) on Beckman Creatinine Analyze 2
Linear Range: 0.5-1.2 mg/dl (0.04-0.11mM)
Specimen requirements: 25 µl of serum in duplicate

Intra-assay variation (C.V.%) = 0.47 (n=6)
Inter-assay variation (C.V.%) = 3.1 (n=3)

Expected values: normal range = 0.6 - 1.59mg/dl

Urinary Creatinine

Supplier: Beckman Diagnostics, Fullerton, CA 92634-3100

Method of Measurement: Jaffe Rate (alkaline picrate) on Beckman Creatinine Analyze 2
Linear Range: 0.2-25 mg/dl (.02-2.2mM)
Specimen requirements: 25 µl of urine in dudplicate

Intra-assay variation (C.V.%) = 1.06 (n=8)
Inter-assay variation (C.V.%) = 1.70 (n=24)

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C-Reactive Protein

ELISA
Supplier: ALPCO, Windham, NH

Linear range: 1.9 - 150 ng/ml
Sensitivity: 0.5 ng/ml
Specimen requirements: 10 µl of serum in duplicate

Intra-assay variation (CV%) = 6.33 (n=4)
Inter-assay variation (CV%) = 2.20 (n=4)

Expected values *

ng/ml

adult/children
< 0.68 - 8.2

new born
< 0.6 ng/ml

pregnant women
< 47 ng/ml

* Values given by kit supplier

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CrossLaps

ELISA
Supplier: Osteometer BioTech

Linear range: 0.040 - 0.219 ng/ml
Sensitivity: 20 ng/ml
Specimen requirements: 50 µl of serum in duplicate

Intra-assay variation (CV%) = 8.15 (n=4)
Inter-assay variation (CV%) = 0.01 (n=2)

Expected values *

1439 - 2159 pM
0.180 - 0.260 ng/ml

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Dehydro Epiandrosterone Sulfate (DHEAS)

Supplier: ALPCO, Windham. NH

Linear range: 0.1 - 10 µg/ml
Sensitivity: 0.02 µg/ml
Specimen requirements: 25 µl of serum/plasma in duplicate

Intra-assay variation (CV%) = 3.28 (n=5)
Inter-assay variation (CV%) = 5.78 (n=11)

Expected values:

men
1.0 - 4.2 µg/ml

women

premenopausal 0.8 - 3.9 µg/ml

postmenopausal 0.1 - 0.6 µg/ml

term pregnancy 0.2 - 1.2 µg/ml

neborns 1.7 - 3.6 µg/ml

men

0 - 30 days premature 0.25 - 10.00 µg/ml

0 - 30 days full term 0.25 - 2.00µg/ml

1 - 11 months n.d.

1 - 6 years < 0.5 µg/ml

>17 years < 6.0 µg/ml

women

0 - 30 days premature 0.25 - 10.00 µg/ml

0 - 30 days full term 0.25 - 2.00µg/ml

1 - 11 months n.d.

1 - 6 years < 0.5 µg/ml

>17 years < 3.0 µg/ml

* Mayo Interpretive Handbook

Conversion factor: 1 µg/ml = 2.6 µM

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Deoxypyridinoline crosslinks (DPD)

ELISA
Supplier: Metra Biosystems, Inc. Palo Alto,CA

Linear range: 3 - 300 nM
Sensitivity: 1.0 nM
Specimen requirements: 50 µl of urine in duplicate

Intra-assay variation (CV%)= 8.44 (n=17)
Inter-assay variation (CV%)= 7.79 (n=72)

Expected values*

men 2.5-5.0 nM/mM creatinine

women 3.0-6.0 nM/mM creatinine

* J. Bone Mineral Res.

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Epinephrine

RIA, by extraction
Supplier: ALPCO, Inc.

Linear range:
Sensitivity: 0.9 ng/ml
Specimen requirements: 1.0 ml EDTA plasma

Intra-assay variation (CV%) = n.d.
Inter-assay variation (CV%) = 3.17 (n=6)

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Estradiol

RIA Supplier: DSL, Inc. Medster, TX
Linear range: 5.0 - 750 pg/ml Sensitivity: 2.2 pg/ml Specimen requirements: 200 µl serum/plasma in duplicate Intra-assay variation (CV%): 4.37 (n=7) Inter-assay variation (CV%): 7.54 (n=5)

Expected values*

group mean range

men 24.6 ± 9.1 pg/ml 5.6 - 50.1

women

follicular

81.7 ± 58.5

37.4 - 201.36

luteal 119.5 ±82.6 34.9 - 374.0

postmenopasual 27.5 ±28.4 5.7 - 102.8
*Mayo Interpretive Handbook

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FSH

Linear range: 2 - 200 mIU/ml Sensitivity: 0.2 mIU/ml Specimen requirements: 100 µl serum/plasma in duplicate Intra-assay variation (CV%): 3.57 (n=16) Inter-assay variation (CV%): 5.76 (n=45)

Expected values*

group
mean mIU/ml mIU/ml range

men
4.6 1.6 - 17.8

women

follicular

6.8

3.6 - 13.7

mid-cycle
13.1 8.2 - 23.4

luteal
4.7 2.3 - 10.7

postmenopasual
57.4 14.8 - 138.8

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Free Fatty Acids, non-esterified

Linear range: 0.25 - 1.00 mEq/L Sensitivity: 0.2 mEq/L Specimen requirements: 25 µl serum in duplicate Intra-assay variation (CV%): 5.73 (n=5) Inter-assay variation (CV%): 4.99 (n=6)

Expected values: 0.1 - 0.6 mEq/L
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Follicle Stimulating Hormone (FSH)

IRMA
Supplier: Nichols Institute Diagnostics , San Juan Capistrano, CA

Linear range: 2-200 mIU/ml
Sensitivity: 0.2 mIU/ml
Specimen requirements: 100 µl of serum in duplicate

Intra-assay variation (CV%)= 5.12 (n=6)
Inter-assay variation (CV%)= 5.78 (n=45)

Expected values *

n Mean mIU/ml Range mIU/ml

males 105 4.6 1.6-17.8

females

follicular 112 6.8 3.6-13.7

Mid cycle 9 13.1 8.2-23.4

Luteal 116 4.7 2.3-10.7

Post menopausal 58 57.4 14.8-138.8

Pre pubertal children 29 1.2 0-9

Values given by kit supplier. Data from literature reports higher values using IRMA assays than the values above (J. Bone Miner. Res. 7(12):1389-1398, 1992.

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Gastric Inhibitory Polypeptide (GIP)

ELISA
Supplier:

Linear range:
Sensitivity:
Specimen Requirements:

Intra-assay variation (CV%) =
Inter-assay variation (CV%) =

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Glucagon-like Peptide (GLP)

ELISA
Supplier:

Linear range:
Sensitivity:
Specimen Requirements:

Intra-assay variation (CV%) =
Inter-assay variation (CV%) =

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Glucose

Glucose Analyzer
Supplier: Beckman Diagnostics, Fullerton, CA 92634-3100

Method of Measurement: Oxygen Rate Method (glucose oxidase) on Beckman Glucose Analyzer 2
Linear Range 1-450 mg/dl
Specimen requirements: 10 µl of serum or urine in duplicate

Intra-assay variation (C.V.%) = 1.38 (n=18)
Inter-assay variation (C.V.%) = 1.52 (n=112)

Expected values

Serum 70-110 mg/dl

Urine 3-25 mg/dl

C.S. Fluid 50-75 mg/dl

Normal Fasting

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Growth Hormone

IRMA
Supplier: Nichols Diagnostics Institute, San Juan Capistrano, CA
Linear Range: 0.5 - 50 ng/ml
Sensitiivity: 0.02 ng/ml Specimen requirements: 100 µl of serum in duplicate

Intra-assay variation (C.V.%) = 8.71 (n=5)
Inter-assay variation (C.V.%) = 0.76 (n=3)

Expected values*

males < 5 ng/ml

females < 10 ng/ml

*Mayo Interpretive Handbook

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25-Hydroxyvitamin D

RIA
Supplier: DiaSorin., Stillwater, Minnesota

Linear range: 5 to 100 ng/ml
Sensitivity: 3.0 ng/ml
Specimen Requirements: 50 µl of serum or plasma (EDTA or heparin) in duplicate

Intra-assay variation (CV%)= 5.19 (n=6)
Inter-assay variation (CV%)= 7.90 (n=12)

Expected values *
Range: 8.9-46.7 ng/ml

* Data provided by INCSTAR.

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ICAM, soluble (sICAM)

ELISA
Supplier: R&D Systems., Minneapolis, MN

Linear range: 2.73 to 49.55 ng/ml
Sensitivity: 0.35 ng/ml
Specimen Requirements: 15 µl of serum or plasma in duplicate

Intra-assay variation (CV%) = 2.48 (n=8)
Inter-assay variation (CV%) = 1.83 (n=5)

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ICTP

RIA
Supplier: DiaSorin., Stillwater, Minnesota

Linear range: 1 to 50 µg/L
Sensitivity: 0.5 µg/L
Specimen Requirements: 100 µl of serum in duplicate

Intra-assay variation (CV%)= 7.84 (n=6)
Inter-assay variation (CV%)= 2.50 (n=2)

Expected values: male = 2.8 µg/L, female = 2.4 µg/L.

Clinical Chemistry 36(7):1328-1332, 1990

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Insulin

RIA
Supplier: Linco Research Inc., St. Louis, MO

Linear range: 2 - 200 mU/ml
Sensitivity: 2mU/ml
Specimen requirements: 100 µl of serum or plasma in duplicate

Intra-assay variation (CV%)= 4.42 (n=73)
Inter-assay variation (CV%)= 5.95 (n=259)

Expected values* Normal fasting 5 - 15 µU/ml (200-600 pg/ml)
* Diabetes 12:116, 1963

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Insulin, Ultra sensitive

RIA, cat # HI-11K
Supplier: Linco Research Inc., St. Louis, MO

Linear range: 0.2 - 20 µU/ml
Sensitivity: 0.2 µU/ml
Specimen requirements: 100 µl of serum or plasma in duplicate
Antibody: guinea pig anti human insulin

Intra-assay variation (CV%)= 2.69 (n=10)
Inter-assay variation (CV%)= 3.27 (n=24)

Expected values*
Normal fasting 5 - 15 µU/ml (200-600 pg/ml)
* Diabetes 12:116, 1963

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Insulin-like Growth Factor-I (IGF-1), acid ethanol extraction

RIA, cat # 40-2100
Supplier: Nichols Institute Diagnostics, San Juan Capistrano, CA

Linear range: 0.3 - 4.8 ng/ml
Sensitivity 0.1 ng/ml
Specimen requirements: 100 µl of serum or EDTA plasma in duplicate

Intra-assay variation (CV%)= 7.15 (n=5)
Inter-assay variation (CV%)= 9.63 (n=23)

Expected values (acid ethanol extraction)*

age n range(ng/ml) mean

males 2-14 25 65.3-841.5 238.9

20-40 47 83.3-378.0 239.1

40+ 19 54.0-328.5 148.6

females 2-14 26 60.8-724.5 296.8

20-40 50 112.5-450.0 236.2

40+ 11 141.8-389.3 217.4

* Data provided by Nichols Institute

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Interferon gamma (IFNg)

ELISA
Supplier: R&D Systems, Minneapolis, MN

Linear range:
Sensitivity:
Sample requirements: 100 µl in duplicate of serum or plasma

Intra-assay variation (CV%)= n.d.
Inter-assay variation (CV%)= n.d.

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Interleukens

IL-6 , high sensitivity

ELISA
Supplier: R&D Systems, Minneapolis, MN

Linear range: 0.156 - 10 pg/ml
Sensitivity: 0.094 pg/ml
Sample requirements: 200 µl in duplicate of serum or plasma

Intra-assay variation (CV%)= n.d.

Inter-assay variation (CV%)= n.d.

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Leptin

RIA, cat # HL-81K
Supplier: Linco Research Inc., St. Louis,MO

Linear range: 0.5-100 ng/ml
Sensitivity: 0.5 ng/ml
Sample requirements: 100 µl in duplicate of serum or plasma

Intra-assay variation (CV%)= 3.85 (n=25)
Inter-assay variation (CV%)= 3.81 (n=51)

Expected values: 1 - 40 ng/ml range
*Dependant on body fat content

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Luteinizing Hormone (LH)

IRMA, cat#40-2190
Supplier: Nichols Institute Diagnostics ,San Juan Capistrano, CA

Linear: 2 - 200 mIU/ml
Sensitivity: 0.1 mIU/ml
Specimen requirements: 100 µl of serum in duplicate

Intra-assay variation (CV%) = 3.37 (n=16)
Inter-assay variation (CV%) = 5.90 (n=65)

Expected values *

n
Mean mIU/ml
Range mIU/ml

males
106
3.0
1.4-11.1

females

follicular
110
4.5
1.9-11.9

Mid cycle
9
35.5
16.8-81.1

Luteal
116
3.6
0.8-17.9

Post menopausal
62
13.4
3.2-33.9

Pre pubertal children
29
-
0-2.7

* Values given by kit supplier. Data from literature reports higher values using IRMA assays than the values given above. J. Bone Miner. Res. 7(12):1389-1398, 1992.

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Lipid Panel

Cholestech LDX
Supplier: Cholestech Corp. Hayward, CA

Linear range:

linear range
sensitivity

total cholesterol 100 - 500 mg/dl
0.8 %

HDL 29 - 72 mg/dl
13 %

LDL 60 - 178 mg/dl
-

VLDL 0 - 40 mg/dl
-

triglycerides 30 - 190 mg/ml
15 %

Ratio TC/HDL 4.0 - 6.7

Specimen requirements: 35 µl serum

Intra-assay variation (CV%) = 3.08 (n=4)
Inter-assay variation (CV%) =3.72 (n=22)

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Norepinephrine

RIA, by extraction
Supplier: ALPCO, Inc.

Linear range:
Sensitivity: 24 pg/ml
Specimen requirements: 1.0 ml EDTA plasma

Intra-assay variation (CV%) =
Inter-assay variation (CV%) = 4.86 (n=4)

Expected values*

supine 70 - 750 pg/ml

standing 200 - 1,700 pg/ml

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NTX (urinary)

ELISA, cat # 9006
Ostex International, Inc.

Linear range: 1.00 to 3000 nM
Sensitivity:
Specimen requirements: 25 µl of urine in duplicate

Intra-assay variation (CV%) = 4.56 (n=4)
Inter-assay variation (CV%) = 8.83 (n=7)

Expected values *

age
n
range

women
25-49
258
14-74 nM BCE/mM

NTX -S (serum)

ELISA, cat # 9021
Ostex International, Inc.

Linear range: 0.00 to 40.00 nM BCE
Sensitivity:
Specimen requirements: 5 µl of serum in duplicate

Intra-assay variation (CV%) = 1.10 (n=5)
Inter-assay variation (CV%) = 0.83 (n=2)

Expected values *

age
n
range

women
25-49
257
6.2 - 19.0 nM BCE

men
31-80
176
5.4 - 24.2 nM BCE

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Osteocalcin

IRMA, cat # 50-1000
Supplier: Nichols Institute Diagnostics,San Juan Capistrano, CA

Linear range: 0.6 to 61 ng/ml
Sensitivity: 0.06 ng/ml
Specimen requirements: 10 µl of serum in duplicate

Intra-assay variation (CV%) = 2.97 (n=20)
Inter-assay variation (CV%) = 6.30 (n=91)

Expected values *

Age n Range (ng/ml)

Men 20-60 49 3.4-11.7

Women 19-58 52 2.4-10.0

* Values given by kit supplier. Data from literature reports higher values using IRMA assays than the values given above. (J. Bone MineralRes. 7(12):1389-1398, 1992)

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Plasminogen Activator Inhibitor/Tissue Plasminogen Activator complex (PAI-1/tPA)

ELISA
Supplier:

Linear range:
Sensitivity:
Specimen Requirements:

Intra-assay variation (CV%) =
Inter-assay variation (CV%) =

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Parathyroid Hormone (intact)

IRMA, cat # 40-2170
Supplier: Nichols Institute Diagnostics,San Juan Capistrano, CA

Linear range: 7.5 to 2000 pg/ml
Sensitivity: 1 pg/ml
Specimen requirements: 200 µl of fasting morning serum or EDTA plasma in duplicate

Intra-assay variation (CV%) = 4.09 (n=5)
Inter-assay variation (CV%) = 4.99 (n=7)

Expected values *

n
Range (pg/ml)

normal
253
10-65

hyperparathyroidism
88
53-1180

hypercacalcemia
63
1-22

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Procollagen Type I Carboxyterminal Propeptide (PICP)

RIA, cat # 35100
Supplier: DiaSorin., Stillwater, Minnesota

Linear range: 25 to 500 ng/ml
Sensitivity: 1.2 ng/ml
Specimen requirements: 100 µl of serum in duplicate

Intra-assay variation (CV%) = 2.24 (n=36)
Inter-assay variation (CV%) = 4.38 (n=77)

Expected values *

Range (ng/ml)

Men
38 - 202

Women
50 - 170

* Clinical Chemistry 36(7):1328-1332, 1990

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Prolactin

IRMA, cat # 40-2165
Supplier: Nichols Diagnostic Institute, Inc., San Juan Capistrano, CA

Linear range: 3 to 155 ng/ml
Sensitivity: 0.14 ng/ml
Specimen requirements: 50 µl of serum in duplicate

Intra-assay variation (CV%) = 2.24 (n=5)
Inter-assay variation (CV%) = 1.52 (n=3)

Expected values *

Range (ng/ml)

Males
3.0 - 14.7

Females, premenopausal
3.8 - 23.2

* Mayo Interpretive Handbook

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sP-Selectin, soluble P-selectin

ELISA
Supplier: R&D Systems, Minneaplois, MN

Linear range: 0.82 - 45.94 ng/ml
Sensitivity: 0.5 ng/ml
Specimen Requirements: 25 µl of serum or plasma

Intra-assay variation (CV%) = 3.64 (n=2)
Inter-assay variation (CV%) = 6.87 (n=5)

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Thyroid Stimulating Hormone (TSH)

IRMA, cat#40-2160
Nichols Institute Diagnostics , San Juan Capistrano, CA

Linear: 0.1 - 100 µIU/ml (1 - 40 ng/ml)
Sensitivity: 0.04 µIU/ml
Specimen requirements: 100 µl of fasting morning serum in duplicate

Intra-assay variation (CV%) = 2.10 (n=5)
Inter-assay variation (CV%) = 5.20 (n=14)

Expected values *

Age n range (µlU/ml)

Adults 20 - 60 192 0.6 - 4.6 µlU/ml

Newborn 1-5 days 49 1.3 - 20.0 µlU/ml

Children 2mo.-14yr. 170 0.6 - 7.4 µlU/ml

* Values given by kit supplier.

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